Media type: E-Article Title: Expression of constitutively active p21H‐rasval12 in postmitotic pyramidal neurons results in increased dendritic size and complexity Contributor: Alpár, Alán; Palm, Kirstin; Schierwagen, Andreas; Arendt, Thomas; Gärtner, Ulrich imprint: Wiley, 2003 Published in: Journal of Comparative Neurology Language: English DOI: 10.1002/cne.10915 ISSN: 0021-9967; 1096-9861 Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>The small G protein p21Ras is a critical molecular switch for relaying neurotrophic actions and is essential for normal functioning and plasticity of the nervous system. In this study, the morphogenetic effects of p21Ras were investigated on neurons in vivo. Morphological changes of layers II/III and Vb commissural pyramidal neurons of the primary somatosensory cortex were analyzed in transgenic mice expressing permanently active p21H‐Ras<jats:sup>Val12</jats:sup> in postmitotic neurons. Pyramidal cells were retrogradely labelled with biotinylated dextran amine and subsequently traced using Neurolucida. Compared with wild‐type mice, transgenic animals showed a significant increase in the surface area and volume of basal dendrites on the proximal and intermediate segments in layers II/III and on further distal segments in layer V. In addition, the surface area and volume of the trunk and of the proximal segments of oblique branches of apical dendrites were enlarged in both layers. Sholl analyses of basal and apical dendrites showed a significant increase in dendritic complexity of layer V neurons. A positive correlation was observed between the size of the basal dendrite and the neuronal soma size in the transgenic population, indicating that growth‐promoting effects of p21H‐Ras<jats:sup>Val12</jats:sup> affect both cellular compartments in parallel. However, the dendritic surface correlated with the number of tips and dendritic stem diameter in both wild‐type and transgenic populations, demonstrating that these relations represent rather conservative design principles in dendritic morphology. The data presented here suggest an important role of p21Ras‐dependent signaling in the final differentiation and maintenance of dendritic morphology. J. Comp. Neurol. 467:119–133, 2003. © 2003 Wiley‐Liss, Inc.</jats:p>