Media type: E-Article Title: Transformation of Recalcitrant Sorghum Varieties Facilitated by Baby Boom and Wuschel2 Contributor: Nelson‐Vasilchik, Kimberly; Hague, Joel; Mookkan, Muruganantham; Zhang, Zhanyuan J.; Kausch, Albert Published: Wiley, 2018 Published in: Current Protocols in Plant Biology, 3 (2018) 4 Language: English DOI: 10.1002/cppb.20076 ISSN: 2379-8068 Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>Most reliable transformation protocols for cereal crops, including sorghum (<jats:italic>Sorghum bicolor</jats:italic> L. Moench), rely on the use of immature embryo explants to generate embryogenic callus cells that are then transformed using <jats:italic>Agrobacterium</jats:italic>‐ or particle‐bombardment‐mediated DNA delivery. Subsequent to DNA transfer, most protocols rely on selectable markers for the recovery of stably transformed callus that is then regenerated to produce T<jats:sub>0</jats:sub> plants. However, these protocols require specific genotypes that are innately capable of efficient embryogenic callus initiation. Here, we describe a system that makes use of the differential expression of the morphogenic regulators <jats:italic>Baby Boom</jats:italic> (<jats:italic>Bbm</jats:italic>) and <jats:italic>Wuschel2</jats:italic> (<jats:italic>Wus2</jats:italic>) to achieve transformation in varieties of sorghum typically recalcitrant to standard transformation methods. © 2018 by John Wiley & Sons, Inc.</jats:p>