Media type: E-Article Title: A novel HoxB cluster protein expressed in the hindbrain and pharyngeal arches Contributor: Saxena, Ankur; Bronner, Marianne E. imprint: Wiley, 2014 Published in: genesis Language: English DOI: 10.1002/dvg.22806 ISSN: 1526-954X; 1526-968X Keywords: Cell Biology ; Endocrinology ; Genetics Origination: Footnote: Description: <jats:title>Summary</jats:title><jats:p>We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a‐Citrine fusion protein is expressed in the developing pharyngeal arches, hindbrain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette‐PCR revealed that CT74a‐Citrine's genomic integration is within the HoxB region, and 3′ RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a‐like pattern. genesis 52:858–863, 2014. © 2014 Wiley Periodicals, Inc.</jats:p>