Montes, Carolina L;
Acosta‐Rodríguez, Eva V;
Mucci, Juan;
Zuniga, Elina I;
Campetella, Oscar;
Gruppi, Adriana
A Trypanosoma cruzi antigen signals CD11b+ cells to secrete cytokines that promote polyclonal B cell proliferation and differentiation into antibody‐secreting cells
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Media type:
E-Article
Title:
A Trypanosoma cruzi antigen signals CD11b+ cells to secrete cytokines that promote polyclonal B cell proliferation and differentiation into antibody‐secreting cells
Contributor:
Montes, Carolina L;
Acosta‐Rodríguez, Eva V;
Mucci, Juan;
Zuniga, Elina I;
Campetella, Oscar;
Gruppi, Adriana
Description:
<jats:title>Abstract</jats:title><jats:p>Microbial‐induced polyclonal activation of B cells is a common event in several forms of infections, and is believed to play a crucial role both for enhancing the production of specific antibodies and for maintenance of B cell memory. Therefore, a major challenge in biomedical research is the identification of pathogen‐derived products capable of rapidly mounting B cell expansion and differentiation. Here we report that glutamate dehydrogenase (GDH) stimulates polyclonal proliferation and differentiation of naive B cells. This stimulation was found to be T cell independent, but to absolutely require CD11b<jats:sup>+</jats:sup> cells. Moreover, we demonstrate that stimulation of CD11b<jats:sup>+</jats:sup> cells by GDH leads to the production of IL‐6, IL‐10 and B cell‐activating factor (BAFF), all of which combine to powerfully induce B cell expansion. Importantly, IL‐6 and IL‐10 further drive B cell terminal differentiation into plasma cells by up‐regulating critical transcription factors and immunoglobulin secretion. Our data provide the first evidence that a protozoan antigen can induce BAFF production by accessory cells, which in concert with other cytokines trigger polyclonal B cell activation.</jats:p>