Description:
AbstractIn vitro amplification of the alphoid repeated sequences clustered in the centromeric regions of the human × and Y chromosomes was carried out. A modification of the amplification conditions reported by Witt and Erickson [1] gave clear amplification bands from 10 pg of genomic DNA template. Results were obtained from the following aged tissue samples: 1 μL three‐year‐old blood stain, one‐year‐old single hair roots, six‐month‐old saliva stains and twelve‐year‐old bone fragments. Good results were obtained even when the template was artificially fragmented into fragments of less than 1 kbp.