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Media type:
E-Article
Title:
Fundamentals and practice for ultrasensitive laser‐induced fluorescence detection in microanalytical systems
Contributor:
Johnson, Mitchell E.;
Landers, James P.
imprint:
Wiley, 2004
Published in:ELECTROPHORESIS
Language:
English
DOI:
10.1002/elps.200406086
ISSN:
0173-0835;
1522-2683
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>Laser‐induced fluorescence is an extremely sensitive method for detection in chemical separations. In addition, it is well‐suited to detection in small volumes, and as such is widely used for capillary electrophoresis and microchip‐based separations. This review explores the detailed instrumental conditions required for sub‐zeptomole, sub‐picomolar detection limits. The key to achieving the best sensitivity is to use an excitation and emission volume that is matched to the separation system and that, simultaneously, will keep scattering and luminescence background to a minimum. We discuss how this is accomplished with confocal detection, 90° on‐capillary detection, and sheath‐flow detection. It is shown that each of these methods have their advantages and disadvantages, but that all can be used to produce extremely sensitive detectors for capillary‐ or microchip‐based separations. Analysis of these capabilities allows prediction of the optimal means of achieving ultrasensitive detection on microchips.</jats:p>