> Details

Kim, Byung‐Soo; Park, Jin Joo; Edler, Lutz; von Fournier, Dietrich; Haase, Wulf; Sautter‐Bihl, Marie‐Luise; Gotzes, Florian; Thielmann, Heinz Walter

New measure of DNA repair in the single‐cell gel electrophoresis (comet) assay

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: New measure of DNA repair in the single‐cell gel electrophoresis (comet) assay
  • Contributor: Kim, Byung‐Soo; Park, Jin Joo; Edler, Lutz; von Fournier, Dietrich; Haase, Wulf; Sautter‐Bihl, Marie‐Luise; Gotzes, Florian; Thielmann, Heinz Walter
  • imprint: Wiley, 2002
  • Published in: Environmental and Molecular Mutagenesis
  • Language: English
  • DOI: 10.1002/em.10090
  • ISSN: 0893-6692; 1098-2280
  • Keywords: Health, Toxicology and Mutagenesis ; Genetics (clinical) ; Epidemiology
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>Since its introduction by Östling and Johanson [1984; Biochem Biophys Res Commun 123:291–298] and independent modifications by Singh et al. [1990; Exp Cell Res 175:184–191] and Olive et al. [1988; Radiat Res 112:86–94], the comet assay has been widely used in genetic toxicology, environmental biomonitoring, molecular and human epidemiology, and clinical investigations. There are still several issues to be resolved before the comet assay is accepted as a standard assay for detecting DNA damage and repair in a single cell. One of the major issues is the proper quantification of DNA damage/repair. The aim of this article is to develop a new quantitative measure of DNA damage/repair which is represented in the dose–time‐response surface. We propose to use the second derivative (2D) of the dose–time‐response surface for measuring DNA repair activity. This approach enables us to represent the DNA repair activity of cells exposed to a DNA‐damaging agent with a single number by combining all the information of a dose–time‐response experiment. The computation procedure includes the application of linear regression. An SAS/AF‐based program, “Comet Assay,” was developed for this computation and is freely available on the Internet. We considered the response of each of four DNA damage parameters: tail moment, tail length, tail DNA, and tail inertia for constructing the dose–time‐response surface. Using data from 25 patients, we observed that 2Ds based on tail moment and tail DNA were highly correlated and that tail inertia might provide information on a somewhat different aspect of DNA damage/repair. Environ. Mol. Mutagen. 40:50–56, 2002. © 2002 Wiley‐Liss, Inc.</jats:p>