Media type: E-Article Title: Prevalence and frequency of circulating t(14;18)‐MBR translocation carrying cells in healthy individuals Contributor: Schüler, Frank; Dölken, Lars; Hirt, Carsten; Kiefer, Thomas; Berg, Tobias; Fusch, Gerhard; Weitmann, K.; Hoffmann, W.; Fusch, Christoph; Janz, Siegfried; Rabkin, Charles S.; Dölken, Gottfried Published: Wiley, 2009 Published in: International Journal of Cancer, 124 (2009) 4, Seite 958-963 Language: English DOI: 10.1002/ijc.23958 ISSN: 0020-7136; 1097-0215 Keywords: Cancer Research ; Oncology Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>The t(14;18) translocation is a common genetic aberration that can be seen as an early step in pathogenesis of follicular lymphoma (FL). The significance of low level circulating t(14;18)‐positive cells in healthy individuals as clonal lymphoma precursors or indicators of risk is still unclear. We determined the age dependent prevalence and frequency of BCL2/IgH rearrangements in 715 healthy individuals ranging from newborns to octo‐ and nonagenarians. These results were compared with number of circulating t(14;18)‐positive cells in 108 FL patients at initial presentation. The overall prevalence of BCL2/IgH junctions in this large sample was 46% (327/715). However, there was a striking dependence upon age. Specifically, among individuals up to 10 years old, none had detectable circulating t(14;18)‐positive cells. In the age groups representing 10–50 years old, we found a steady elevation in the prevalence of BCL2/IgH junctions up to a prevalence of 66%. Further increases of the prevalence in individuals older than 50 years were not seen. The mean frequency of BCL2/IgH junctions in healthy individuals ≥40 years (18–26 × 10<jats:sup>−6</jats:sup>) was significantly higher than in younger subjects (7–9 × 10<jats:sup>−6</jats:sup>). Four percent (31/715) of individuals carried more than one t(14;18)‐positive cell per 25,000 peripheral blood mononuclear cells (PBMNCs). In comparison, 108 stage III/IV FL patients had a median number of circulating t(14;18)‐positive malignant FL cells of about 9200/1 million PBMNCs (range 7–1,000,000). These findings will further improve the understanding of the relevance of t(14;18)‐positive cells in healthy individuals as a risk marker toward the development into lymphoma precursors. © 2008 Wiley‐Liss, Inc.</jats:p> Access State: Open Access