Description:
AbstractTwo tumor‐specific antigens, with molecular weights of approximately 82 and 86 kDa, have been isolated and purified to apparent homogeneity from the methylcholanthrene‐induced sarcoma, CII‐7. The method of purification used was essentially that previously employed for the isolation of the 82‐ and 86‐kDa antigens from the Meth A sarcoma and the 86‐kDa antigen from mKSA sarcoma. Cytosolic fractions were subjected to hexylamine agarose chromatography, Sepharose S‐300 filtration and hydroxylapatite chromatography. A final step, HPLC‐DEAE chromatography, was necessary for the purification of the 82‐kDa protein. Both isolated antigens retained their specific immunogenicity for CII‐7 as determined by in vivo tumor rejection assays, and failed to influence the growth of other syngeneic sarcomas, CI‐4, CII‐10 and mKSA, which have their own unique TATA. The 82‐and 86‐kDa antigens appear to be distinct proteins that are well conserved in nature and may represent members of distinct families of TATA.