Hodgson, María Isabel;
Pantoja, Humberto;
Latorre, Juan Jose;
Vial, Pablo;
Henríquez, Alejandra;
Wenger, Jonny;
Peña, Alfredo;
Siri, maría Teresa;
Guiraldes, Ernesto
Helicobacter pylori‐Associated Gastroduodenal Disease in Symptomatic Chilean Children : Diagnostic Value of Serological Assay
: Diagnostic Value of Serological Assay
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Media type:
E-Article
Title:
Helicobacter pylori‐Associated Gastroduodenal Disease in Symptomatic Chilean Children : Diagnostic Value of Serological Assay
:
Diagnostic Value of Serological Assay
Contributor:
Hodgson, María Isabel;
Pantoja, Humberto;
Latorre, Juan Jose;
Vial, Pablo;
Henríquez, Alejandra;
Wenger, Jonny;
Peña, Alfredo;
Siri, maría Teresa;
Guiraldes, Ernesto
imprint:
Wiley, 1995
Published in:Journal of Pediatric Gastroenterology and Nutrition
Description:
<jats:sec><jats:title>Summary:</jats:title><jats:p>A newly developed enzyme‐linked immuno‐sorbent assay (ELISA) IgG serological assay for the diagnosis of <jats:italic>Helicobacter pylori</jats:italic> infection was used recently in two epidemiological surveys in Chile. To evaluate the diagnostic efficacy of this assay in a local symptomatic pediatric population, we studied 70 school‐age patients referred for upper gastrointestinal endoscopy because of complaints suggestive of gastroduodenal disease. Evidence for antral <jats:italic>H. pylori</jats:italic> infection was sought by three biopsy‐related methods: culture, histology, and urease activity. IgG anti‐<jats:italic>H. pylori</jats:italic> serum antibodies were determined by ELISA. Altogether, chronic antral gastritis was found in 55 patients and duodenal ulcers in nine; 11 subjects had normal histology. Sixty (86%) patients had <jats:italic>H. pylori</jats:italic> in the antrum. This group had significantly higher mean IgG optical density values when compared with the H. pylori‐negative group (1.860 versus 0.669; p < 0.001). The sensitivity and specificity of the assay in detecting antral <jats:italic>H. pylori</jats:italic> were both 90% the positive predictive value was 98% and the negative, 60%. Accuracy of the assay was superior in predicting the presence or absence of gastroduodenal lesions with a sensitivity of 96%, a specificity of 92%, a positive predictive value of 98%, and a negative predictive value of 86%. We conclude that the diagnostic efficiency of this assay renders it appropriate both to screen for <jats:italic>H. Pylori</jats:italic>‐associated gastroduodenal disease in individual patients and to be used in seroepi‐demiological surveys.</jats:p></jats:sec>