> Details

des Rieux, Anne; De Berdt, Pauline; Ansorena, Eduardo; Ucakar, Bernard; Damien, Jacobs; Schakman, Olivier; Audouard, Emilie; Bouzin, Caroline; Auhl, Dietmar; Simón‐Yarza, Teresa; Feron, Olivier; Blanco‐Prieto, Maria J.; Carmeliet, Peter; Bailly, Christian; Clotman, Fréderic; Préat, Véronique

Vascular endothelial growth factor‐loaded injectable hydrogel enhances plasticity in the injured spinal cord

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Vascular endothelial growth factor‐loaded injectable hydrogel enhances plasticity in the injured spinal cord
  • Contributor: des Rieux, Anne; De Berdt, Pauline; Ansorena, Eduardo; Ucakar, Bernard; Damien, Jacobs; Schakman, Olivier; Audouard, Emilie; Bouzin, Caroline; Auhl, Dietmar; Simón‐Yarza, Teresa; Feron, Olivier; Blanco‐Prieto, Maria J.; Carmeliet, Peter; Bailly, Christian; Clotman, Fréderic; Préat, Véronique
  • imprint: Wiley, 2014
  • Published in: Journal of Biomedical Materials Research Part A
  • Language: English
  • DOI: 10.1002/jbm.a.34915
  • ISSN: 1552-4965; 1549-3296
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>We hypothesized that vascular endothelial growth factor (VEGF)‐containing hydrogels that gelify <jats:italic>in situ</jats:italic> after injection into a traumatized spinal cord, could stimulate spinal cord regeneration. Injectable hydrogels composed of 0.5% Pronova UPMVG MVG alginate, supplemented or not with fibrinogen, were used. The addition of fibrinogen to alginate had no effect on cell proliferation <jats:italic>in vitro</jats:italic> but supported neurite growth <jats:italic>ex vivo</jats:italic>. When injected into a rat spinal cord in a hemisection model, alginate supplemented with fibrinogen was well tolerated. The release of VEGF that was incorporated into the hydrogel was influenced by the VEGF formulation [encapsulated in microspheres or in nanoparticles or in solution (free)]. A combination of free VEGF and VEGF‐loaded nanoparticles was mixed with alginate:fibrinogen and injected into the lesion of the spinal cord. Four weeks post injection, angiogenesis and neurite growth were increased compared to hydrogel alone. The local delivery of VEGF by injectable alginate:fibrinogen‐based hydrogel induced some plasticity in the injured spinal cord involving fiber growth into the lesion site. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2345–2355, 2014.</jats:p>