Media type: E-Article Title: Proteinase inhibitors from Streptomyces with antiviral activity Contributor: Serkedjieva, Julia; Angelova, Lidiya; Remichkova, Mimi; Ivanova, Iskra imprint: Wiley, 2006 Published in: Journal of Basic Microbiology Language: English DOI: 10.1002/jobm.200510127 ISSN: 0233-111X; 1521-4028 Keywords: Applied Microbiology and Biotechnology ; General Medicine Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>An extensive screening study for the production of proteolytic inhibitors has been carried out on 75 <jats:italic>Streptomyces</jats:italic> strains. It was found that 18 of the strains and/or their variants (24%) produced proteinaceous substances, which belonged to the group of typical serine protease inhibitors. 23 samples were tested for inhibitory activity on the replication of influenza virus A/Germany/34, strain Rostock (H7N1) (A/Rostock) in chicken embryonic fibroblast (CEF) cells. Eleven of the tested samples (52.2%) significantly inhibited viral growth. Further the specific inhibitory effect on the replication of influenza virus A/Aichi/2/68 (H3N2) (A/Aichi) in Madin‐Darby canine kidney (MDCK) cells and on the growth of herpes simplex virus type 1, strain DA (HSV‐1) in Madin‐Darby bovine kidney (MDBK) cells was tested. Nine samples significantly inhibited A/Aichi and four – HSV‐1. The most effective inhibitors, produced by <jats:italic>Streptomyces</jats:italic> sp. 225b (SS 225b) and <jats:italic>Streptomyces chromofuscus</jats:italic> 34‐1 (SS 34‐1) protected mice from mortality in the experimental influenza A/Aichi virus infection. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</jats:p>