Media type: E-Article Title: Impact of knee joint loading on fragmentation of serum cartilage oligomeric matrix protein Contributor: Firner, Sara; Zaucke, Frank; Heilig, Juliane; de Marées, Markus; Willwacher, Steffen; Brüggemann, Gert‐Peter; Niehoff, Anja imprint: Wiley, 2020 Published in: Journal of Orthopaedic Research Language: English DOI: 10.1002/jor.24586 ISSN: 0736-0266; 1554-527X Keywords: Orthopedics and Sports Medicine Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>The aim of the study was to examine the effect of mechanical knee joint loading on the fragmentation pattern of serum cartilage oligomeric matrix protein (COMP). Ten healthy men ran with knee orthoses that were passive or active (+30.9 N·m external flexion moments) on a treadmill (30 minute; <jats:italic>v</jats:italic> = 2.2 m/s). Lower‐limb mechanics, serum COMP levels, and fragmentation patterns (baseline; 0, 0.5, 1, 2 hours postrunning) were analyzed. Running with active orthoses enhanced knee flexion moments, ankle dorsiflexion, and knee flexion angles (<jats:italic>P</jats:italic> < .05). There was an increase in serum COMP (+25%; pre: 8.9 ± 2.4 U/l; post: 10.7 ± 1.9 U/l, <jats:italic>P</jats:italic> = .001), COMP pentamer/tetramer (+88%; 1.88 ± 0.81, <jats:italic>P</jats:italic> = .007), trimer (+209%; 3.09 ± 2.65, <jats:italic>P</jats:italic> = .005), and monomer (+78%; 1.78 ± 0.85, <jats:italic>P</jats:italic> = .007) after running with passive orthoses and in serum COMP (+41%; pre: 8.5 ± 2.7 U/l; post: 11.3 ± 2.1 U/l, <jats:italic>P</jats:italic> < .001), COMP pentamer/tetramer (+57%; 1.57 ± 0.39, <jats:italic>P</jats:italic> = .007), trimer (+86%; 1.86 ± 0.47, <jats:italic>P</jats:italic> = .005), and monomer (+19%; 1.19 ± 0.34, <jats:italic>P</jats:italic> = .114) after running with active orthoses. Increased fragmentation might indicate COMP release from cartilage while running. Interestingly, 0.5 h up to 2 hours after running with passive orthoses, trimer (0.5 hour: 2.73 ± 3.40, <jats:italic>P</jats:italic> = .029; 2 hours: 2.33 ± 2.88, <jats:italic>P</jats:italic> = .037), and monomer (0.5 hour: 2.23 ± 2.33, <jats:italic>P</jats:italic> = .007; 1 hour: 2.55 ± 1.96, <jats:italic>P</jats:italic> = .012; 2 hours: 2.65 ± 2.50, <jats:italic>P</jats:italic> = .009) increased while after running with active orthoses, pentamer/tetramer (1 hour: 0.79 ± 0.28, <jats:italic>P</jats:italic> = .029), and trimer (1 hour: 0.63 ± 0.14, <jats:italic>P</jats:italic> = .005; 2 hours: 0.68 ± 0.34, <jats:italic>P</jats:italic> = .047) decreased. It seems that COMP degradation and clearance vary depending on joint loading characteristics.</jats:p>