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Media type:
E-Article
Title:
MHC class II-dependent activation of CD4+ T cell hybridomas by human mast cells through superantigen presentation
Contributor:
Poncet, Pascal;
Arock, Michel;
David, Bernard
Published:
Oxford University Press (OUP), 1999
Published in:
Journal of Leukocyte Biology, 66 (1999) 1, Seite 105-112
Language:
English
DOI:
10.1002/jlb.66.1.105
ISSN:
0741-5400;
1938-3673
Origination:
Footnote:
Description:
Abstract Human mast cells (MC) were examined for expression of MHC class II antigens and for their ability to activate CD4+ T cell hybridomas through presentation of superantigen (SAg). HMC-1, a leukemic immature MC line expressing class II Ags, was shown to efficiently present the staphylococcal enterotoxin B (SEB) SAg to responding T cell hybridoma on treatment with interferon-γ (IFN-γ), which up-regulated class II molecules. The study was then extended to human normal MC. Almost pure (>99%) cord blood-derived MC (CBMC) were shown to express class II Ags (HLA-Dr and HLA-DQ) and CD80, which were up-regulated by IFN-γ treatment and, to a lesser extent, by interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF). CBMC directly activated CD4+ T cell hybridomas through presentation of SEB and TSST1 SAgs. The production of IL-2 required a cell-to-cell contact between T cells and CBMC and it was inhibited by anti-class II antibodies. Furthermore, an additional pretreatment of CBMC by IFN-γ or GM-CSF or IL-4 had no effect on their presenting efficiency. This previously unknown function of human MC, i.e., MHC class II-dependent activation of CD4+ T cells, may be critical in subsequent cellular activation events because colocalization of mast and T cells is frequently observed at sites of antigen entry. J. Leukoc. Biol. 66: 105–112; 1999.