Description:
<jats:title>Abstract</jats:title><jats:p>A novel Headspace Solid Phase Microextraction (HS‐SPME) protocol is proposed for the analysis of floral scent. Volatile compounds emitted from the flower are collected on a Carboxen/PDMS fiber for 1 hour, transferred to the GC, and analyzed by GC/MS. The method completely eliminates the use of organic solvents, does not require special instrumentation, and may readily be performed in the field without access to mains electricity and other energy supplies. The method is robust, sensitive, and reduces the sampling stress on the investigated plant. Since enzymatic reactions in living flowers may cause changes in the composition of emitted fragrance, dried rosemary (<jats:italic>Rosmarinus officinalis</jats:italic> L.) was used as a stable standard for the method development and optimization. In addition, grape wine was also suggested as homogeneous, bio‐compatible, and relatively stable standard of pronounced and typical scent for the same purpose. The optimized method was used for the comparative investigation of the fragrances emitted by two different species – <jats:italic>Lathyrus vernus</jats:italic> (L.) and <jats:italic>Orchis pallens</jats:italic> (L.). Several monoterpenes (C10 compounds) were found as the main fragrance components of lathyrus, while sesquiterpenes (C15 compounds) were typical for the orchid.</jats:p>