Media type: E-Article Title: Temporal changes in gene expression induced by sulforaphane in human prostate cancer cells Contributor: Bhamre, Suvarna; Sahoo, Debashis; Tibshirani, Robert; Dill, David L.; Brooks, James D. Published: Wiley, 2009 Published in: The Prostate, 69 (2009) 2, Seite 181-190 Language: English DOI: 10.1002/pros.20869 ISSN: 0270-4137; 1097-0045 Origination: Footnote: Description: AbstractBACKGROUNDProstate cancer is thought to arise as a result of oxidative stresses and induction of antioxidant electrophile defense (phase 2) enzymes has been proposed as a prostate cancer prevention strategy. The isothiocyanate sulforaphane, derived from cruciferous vegetables like broccoli, potently induces surrogate markers of phase 2 enzyme activity in prostate cells in vitro and in vivo. To better understand the temporal effects of sulforaphane and broccoli sprouts on gene expression in prostate cells, we carried out comprehensive transcriptome analysis using cDNA microarrays.METHODSTranscripts significantly modulated by sulforaphane over time were identified using StepMiner analysis. Ingenuity Pathway Analysis (IPA) was used to identify biological pathways, networks, and functions significantly altered by sulforaphane treatment.RESULTSStepMiner and IPA revealed significant changes in many transcripts associated with cell growth and cell cycle, as well as a significant number associated with cellular response to oxidative damage and stress. Comparison to an existing dataset suggested that sulforaphane blocked cell growth by inducing G2/M arrest. Cell growth assays and flow cytometry analysis confirmed that sulforaphane inhibited cell growth and induced cell cycle arrest.CONCLUSIONSOur data suggest that in prostate cells sulforaphane primarily induces cellular defenses and inhibits cell growth by causing G2/M phase arrest. Furthermore, based on the striking similarities in the gene expression patterns induced across experiments in these cells, sulforaphane appears to be the primary bioactive compound present in broccoli sprouts, suggesting that broccoli sprouts can serve as a suitable source for sulforaphane in intervention trials. Prostate 69: 181–190, 2009. © 2008 Wiley–Liss, Inc.