Media type: E-Article Title: Visualizing fungicide action: anin vivotool for rapid validation of fungicides with target location HOG pathway Contributor: Bohnert, Stefan; Neumann, Hendrik; Thines, Eckhard; Jacob, Stefan Published: Wiley, 2019 Published in: Pest Management Science, 75 (2019) 3, Seite 772-778 Language: English DOI: 10.1002/ps.5177 ISSN: 1526-498X; 1526-4998 Origination: Footnote: Description: AbstractBACKGROUNDThe mitogen‐activated protein kinase MoHog1p was fused with a green fluorescent protein (GFP) in the filamentous fungusMagnaporthe oryzae. TheMoHOG1::GFPmutant was found to be an excellent tool visualizingin vivofungicide‐dependent translocation of MoHog1p into the nucleus. Validation of pathway specificity was achieved by generating fluorescence‐labelled MoHog1p in theΔMohik1‘loss of function’ mutant strain.RESULTSGFP‐labelled MoHog1p expressed in the wildtype and inΔMohik1demonstrates that fludioxonil is acting on the HOG pathway and even more precisely that fungicide action is dependent on the group III histidine kinase MoHik1p. GFP‐tagged MoHog1p translocated into the nucleus upon fungicide treatment in theMoHOG1::GFPmutant within seconds, but did not do so in theΔMohik1/HOG1::GFPmutant.CONCLUSIONHere, we developed a rapidin vivotool for fluorescent‐based validation of fungicides targeting the HOG‐signaling pathway. Furthermore, using the fluorescent mutants generated in this study, we are able to visualize that fungicide action is dependent on the histidine kinase MoHik1p but operates in a different mechanism of pathway activation compared to osmotic stress. © 2018 Society of Chemical Industry