> Details

Gupta, Prachi; Bansal, Mohinder Pal; Koul, Ashwani

Spectroscopic Characterization of Lycopene Extract from Lycopersicum esculentum (Tomato) and Its Evaluation as a Chemopreventive Agent Against Experimental Hepatocarcinogenesis in Mice

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Spectroscopic Characterization of Lycopene Extract from Lycopersicum esculentum (Tomato) and Its Evaluation as a Chemopreventive Agent Against Experimental Hepatocarcinogenesis in Mice
  • Contributor: Gupta, Prachi; Bansal, Mohinder Pal; Koul, Ashwani
  • Published: Wiley, 2013
  • Published in: Phytotherapy Research, 27 (2013) 3, Seite 448-456
  • Language: English
  • DOI: 10.1002/ptr.4741
  • ISSN: 0951-418X; 1099-1573
  • Origination:
  • Footnote:
  • Description: The present study was designed to characterize the lycopene extract (LycT) prepared from tomatoes (Lycopersicum esculentum) and then to evaluate its chemopreventive efficacy in N‐diethylnitrosamine (NDEA)‐induced experimental hepatocarcinogenesis in female Balb/c mice. The extraction of lycopene was carried out using hexane/acetone/ethanol as an extracting medium and then characterized by ultraviolet–visible, nuclear magnetic resonance and Fourier transform infrared spectroscopy. Chemopreventive efficacy of characterized LycT in vivo was evaluated in terms of hepatic tumour incidence, multiplicity, burden, hepatosomatic index and animal survival rate. Results indicated that average lycopene content of the tomato was 11.6–14 mg/kg tomato weight. Spectroscopic data confirmed the structural characteristics of lycopene in the extract. In the animal study, reduction in tumour incidence (42.05%), tumour burden (1.39) and tumour multiplicity (3.42) was observed upon LycT pretreatment to NDEA‐treated animals. Histopathological analysis unravelled that the increased survival rate in LycT + NDEA‐treated animals was due to the delay in the formation of aggressive tumour nodules. These observations indicate that lycopene seems to be an able candidate for chemoprevention in hepatocarcinogenesis resulting from NDEA insults. Copyright © 2012 John Wiley & Sons, Ltd.