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Media type:
E-Article
Title:
ORGANIZATION OF FOCAL ADHESION PLAQUES IS DISRUPTED BY ACTION OF THE HIV‐1 PROTEASE
Contributor:
Shoeman, Robert L.;
Hartig, Roland;
Hauses, Claudia;
Traub, Peter
Published:
Wiley, 2002
Published in:
Cell Biology International, 26 (2002) 6, Seite 529-539
Language:
English
DOI:
10.1006/cbir.2002.0895
ISSN:
1065-6995;
1095-8355
Origination:
Footnote:
Description:
AbstractFocal adhesion plaques were severely affected in human embryonic fibroblasts permeabilized with digitonin and incubated in buffer containing the human immunodeficiency virus type 1 protease (HIV‐1 PR). A mutant HIV‐1 PR (3271 HIV‐1 PR) had no effect on focal adhesion plaques. Similar effects were seen with cells microinjected with either HIV‐1 PR or 3271 HIV‐1 PR. Immunoblots of the human embryonic fibroblasts demonstrated that a number of focal adhesion plaque proteins were specifically cleaved by HIV‐1 PR. These included fimbrin, focal adhesion plaque kinase (FAK), talin, and, to a lesser extent, filamin, spectrin and fibronectin. Proteins detected by antibodies to β4 integrin and α3 integrin were also cleaved by the HIV‐1 PR. Control experiments demonstrated that the effect and protein cleavages described are due to action of the HIV‐1 PR and not to the action of endogenous host cell proteases.