• Media type: E-Article
  • Title: Fatty acid selectivity of lipases: γ‐linolenic acid from borage oil
  • Contributor: Foglia, Thomas A.; Sonnet, Philip E.
  • imprint: Wiley, 1995
  • Published in: Journal of the American Oil Chemists' Society
  • Language: English
  • DOI: 10.1007/bf02636081
  • ISSN: 0003-021X; 1558-9331
  • Keywords: Organic Chemistry ; General Chemical Engineering
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>The γ‐linolenic acid (<jats:italic>Z,Z,Z</jats:italic>‐6,9,12‐octadecatrienoic acid, GLA) present in borage oil free fatty acids was concentrated in esterification reactions that were catalyzed by several preparations of the acyl‐specific lipase of<jats:italic>Geotrichum candidum</jats:italic>. In this manner, a 95% recovery of the GLA originally present in borage oil (25% GLA) was obtained as a highly enriched fatty acid fraction with a GLA content of &gt;70%. Other fatty acids concentrated in this fraction were the monounsaturated fatty acids with chainlengths of C‐20 and longer that were present in the oil. An immobilized preparation of<jats:italic>G. candidum</jats:italic> on silica gel also was used for the enrichment of GLA in borage oil. In this instance, a 75% recovery of GLA was obtained, and the supported lipase was reusable (three cycles) with minimal loss in activity.</jats:p>