You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Changes in the lipid profile of hamster liver after Schistosoma mansoni infection, characterized by mass spectrometry imaging and LC–MS/MS analysis
Contributor:
Wiedemann, Katja R.;
Peter Ventura, Alejandra;
Gerbig, Stefanie;
Roderfeld, Martin;
Quack, Thomas;
Grevelding, Christoph G.;
Roeb, Elke;
Spengler, Bernhard
imprint:
Springer Science and Business Media LLC, 2022
Published in:Analytical and Bioanalytical Chemistry
Language:
English
DOI:
10.1007/s00216-022-04006-6
ISSN:
1618-2642;
1618-2650
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>Schistosomiasis, caused by the human parasite<jats:italic>Schistosoma mansoni</jats:italic>, is one of the WHO-listed neglected tropical diseases (NTDs), and it has severe impact on morbidity and mortality, especially in Africa. Not only the adult worms but also their eggs are responsible for health problems. Up to 50% of the eggs produced by the female worms are not excreted with the feces but are trapped in the host tissue, such as the liver, where they provoke immune responses and a change in the lipid profile. We built up a database with 372 infection markers found in livers of<jats:italic>S. mansoni</jats:italic>-infected hamsters, using LC–MS/MS for identification, followed by statistical analysis. Most of them belong to the lipid classes of phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and triglycerides (TGs). We assigned some of these markers to specific anatomical structures by applying high-resolution MALDI MSI to cryosections of hamster liver and generating ion images based on the marker list from the LC–MS/MS experiments. Furthermore, enrichment and depletion of several markers were visualized.</jats:p><jats:p><jats:bold>Graphic Abstract</jats:bold></jats:p>