Mall, Julian W.;
Schwenk, Wolfgang;
Philipp, Andreas W.;
Büttemeyer, Rolf;
Pollmann, Christian
Intraperitoneal Administration of the Angiogenesis Inhibitor Thalidomide Does Not Impair Anastomotic Healing following Large Bowel Resection in a Rabbit Model
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Media type:
E-Article
Title:
Intraperitoneal Administration of the Angiogenesis Inhibitor Thalidomide Does Not Impair Anastomotic Healing following Large Bowel Resection in a Rabbit Model
Contributor:
Mall, Julian W.;
Schwenk, Wolfgang;
Philipp, Andreas W.;
Büttemeyer, Rolf;
Pollmann, Christian
Published:
Wiley, 2003
Published in:
World Journal of Surgery, 27 (2003) 10, Seite 1119-1123
Description:
<jats:title>Abstract</jats:title><jats:p>The effects of thalidomide after intraperitoneal instillation on the healing of colonic anastomoses are not known. A series of 40 New Zealand White rabbits underwent an end‐to‐end colonic anastomosis. The animals were randomized into four groups. Groups 1 (<jats:italic>n</jats:italic> = 10) and 2 (<jats:italic>n</jats:italic> = 10) were treated with dissolved thalidomide 200 mg/kg intraperitoneally, whereas groups 3 (<jats:italic>n</jats:italic> = 10) and 4 (<jats:italic>n</jats:italic> = 10) were treated only with the dissolver. Animals were sacrificed at day 3 (groups 1, 3) and day 7 (groups 2, 4). Anastomotic healing was tested by measuring the bursting pressure in vitro. Immunohistochemical staining of the anastomotic site was performed with polyclonal antibodies against CD31 and Mib‐1, to determine a possible antiangiogenic or antiproliferative effect. Statistical analysis was performed using Spearman’s log rank correlation and paired <jats:italic>t</jats:italic>‐test. On postoperative day 3 (<jats:italic>p</jats:italic> > 0.19) and postoperative day 7 (<jats:italic>p</jats:italic> > 0.73), there was no difference in bursting pressure in the treatment and the control groups. Angiogenesis scores were reduced at day 3 in the thalidomide group (<jats:italic>p</jats:italic> < 0.05), but did not differ between the groups at day 7. White blood cell counts were decreased in the treatment groups at day 3 (<jats:italic>p</jats:italic> < 0.01) and day 7, compared to control groups (<jats:italic>p</jats:italic> < 0.01). There was no difference in the expression of Mib‐1 in either group at day 3 or day 7. The intraperitoneal administration of thalidomide does not interfere with the healing of colonic anastomosis. Although the angiogenesis score is diminished at day 3, this did not lead to a reduced bursting pressure at day 3 or day 7.</jats:p>