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Media type:
E-Article
Title:
Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)
Contributor:
Blanes-García, Marta;
Marinović, Zoran;
Herranz-Jusdado, Juan Germán;
Xie, Xuan;
Ferrão, Leonor;
Gallego, Victor;
Pérez, Luz;
Baloch, Abdul Rasheed;
Horváth, Ákos;
Pšenička, Martin;
Asturiano, Juan F.;
Morini, Marina
imprint:
Springer Science and Business Media LLC, 2024
Published in:Fish Physiology and Biochemistry
Language:
English
DOI:
10.1007/s10695-024-01338-1
ISSN:
1573-5168;
0920-1742
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (<jats:italic>Anguilla anguilla</jats:italic>) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: <jats:italic>vasa</jats:italic>, <jats:italic>nanos2</jats:italic>, and <jats:italic>dnd1</jats:italic>. Two <jats:italic>vasa</jats:italic> (<jats:italic>vasa1</jats:italic> and <jats:italic>vasa2</jats:italic>) genes, <jats:italic>nanos2</jats:italic>, and <jats:italic>dnd1</jats:italic> were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that <jats:italic>vasa1</jats:italic> and <jats:italic>vasa2</jats:italic> had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, <jats:italic>vasa1</jats:italic> and <jats:italic>vasa2</jats:italic> exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, <jats:italic>vasa1</jats:italic> and <jats:italic>vasa2</jats:italic> were found to be the most effective markers for spermatogonial cells in the European eel.</jats:p>