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Hackett, Jim; Misra, Rajeev; Reeves, Peter

The TolC protein of Escherichia coli K12 is synthesised in a precursor form

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  • Media type: E-Article
  • Title: The TolC protein of Escherichia coli K12 is synthesised in a precursor form
  • Contributor: Hackett, Jim; Misra, Rajeev; Reeves, Peter
  • imprint: Wiley, 1983
  • Published in: FEBS Letters
  • Language: English
  • DOI: 10.1016/0014-5793(83)80518-3
  • ISSN: 0014-5793; 1873-3468
  • Keywords: Cell Biology ; Genetics ; Molecular Biology ; Biochemistry ; Structural Biology ; Biophysics
  • Origination:
  • Footnote:
  • Description: <jats:p>We examined the biosynthesis of the TolC protein of <jats:italic>Escherichia coli</jats:italic> K12 in a pulse—chase experiment, followed by immunoprecipitation with anti‐TolC antibody and SDS—PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form (<jats:italic>M</jats:italic> <jats:sub>r</jats:sub> 54 500) which could be chased into the mature form (<jats:italic>M</jats:italic> <jats:sub>r</jats:sub> 52 000). DNA sequencing of a portion of the cloned <jats:italic>tolC</jats:italic> gene showed that the N‐terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (<jats:italic>M</jats:italic> <jats:sub>r</jats:sub> 2542). The initiator Met was preceded by a Shine—Dalgarno sequence, with the correct spacing.</jats:p>
  • Access State: Open Access