Isolation and expansion of equine umbilical cord‐derived matrix cells (EUCMCs)

Media type: E-Article
Title: Isolation and expansion of equine umbilical cord‐derived matrix cells (EUCMCs)
Contributor: Passeri, Simona; Nocchi, Francesca; Lamanna, Roberta; Lapi, Simone; Miragliotta, Vincenzo; Giannessi, Elisabetta; Abramo, Francesca; Stornelli, Maria Rita; Matarazzo, Micheletino; Plenteda, Daniele; Urciuoli, Patrizia; Scatena, Fabrizio; Coli, Alessandra
imprint: Wiley, 2009
Published in: Cell Biology International
Language: English
DOI: 10.1016/j.cellbi.2008.10.012
ISSN: 1095-8355; 1065-6995
Keywords: Cell Biology ; General Medicine
Origination:
Footnote:
Description: <jats:title>Abstract</jats:title><jats:p>Stem cells from extra‐embryonic sources can be obtained by non‐invasive procedures. We have standardized a method for the expansion of equine umbilical cord‐derived matrix cells (EUCMCs) for potential therapy.</jats:p><jats:p>EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in α‐MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to α‐MEM.</jats:p><jats:p>α‐MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of α‐MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol.</jats:p><jats:p>Both α‐MEM and MSCBM can be used to cultivate EUCMCs. α‐MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since α‐MEM is more cost‐effective than MSCBM.</jats:p>

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