• Media type: E-Article
  • Title: Multimerization potential of the cytoplasmic domain of the human cytomegalovirus glycoprotein B
  • Contributor: Scheffczik, Hanno; Kraus, Ina; Kiermayer, Simone; Bogner, Elke; Holzenburg, Andreas; Garten, Wolfgang; Eickmann, Markus
  • Published: Wiley, 2001
  • Published in: FEBS Letters, 506 (2001) 2, Seite 113-116
  • Language: English
  • DOI: 10.1016/s0014-5793(01)02891-5
  • ISSN: 0014-5793; 1873-3468
  • Keywords: Cell Biology ; Genetics ; Molecular Biology ; Biochemistry ; Structural Biology ; Biophysics
  • Origination:
  • Footnote:
  • Description: In the present study the coding sequence of the cytoplasmic tail of the human cytomegalovirus glycoprotein B (gB) was expressed. The secondary structure of the purified recombinant protein was analyzed by circular dichroism, and the quaternary structure was investigated by gel permeation chromatography, and electron microscopy. Our data indicate that the cytoplasmic gB domain contains α‐helix structures and assembles into tetramers, suggesting that the authentic gB may represent a homotetramer.
  • Access State: Open Access