Published in:
FEBS Letters, 402 (1997) 2-3, Seite 162-166
Language:
English
DOI:
10.1016/s0014-5793(96)01517-7
ISSN:
0014-5793;
1873-3468
Origination:
Footnote:
Description:
<jats:p>Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis‐type 12‐lipoxygenase (<jats:italic>e12</jats:italic>‐lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene <jats:italic>Aloxe</jats:italic>, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4–8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12(<jats:italic>S</jats:italic>)‐HETE from arachidonic acid. By fluorescence in situ hybridization the <jats:italic>e12</jats:italic>‐lipoxygenase gene was localized to chromosome band 11 B1–B3.</jats:p>