• Media type: E-Article
  • Title: Isolation by differential display and characterization of a tobacco auxin‐responsive cDNA Nt‐gh3, related to GH3
  • Contributor: Roux, Camille; Perrot-Rechenmann, Catherine
  • Published: Wiley, 1997
  • Published in: FEBS Letters, 419 (1997) 1, Seite 131-136
  • Language: English
  • DOI: 10.1016/s0014-5793(97)01447-6
  • ISSN: 0014-5793; 1873-3468
  • Keywords: Cell Biology ; Genetics ; Molecular Biology ; Biochemistry ; Structural Biology ; Biophysics
  • Origination:
  • Footnote:
  • Description: <jats:p>By use of differential display, we have isolated a new early auxin‐responsive cDNA in <jats:italic>Nicotiana tabacum</jats:italic>. <jats:italic>Nt‐gh3</jats:italic> had 70% identity with the unique <jats:italic>GH3</jats:italic> sequence isolated in soybean by Hagen et al. [Planta 162 (1984) 147–153] and is thus the first reported cDNA related to this gene until now. <jats:italic>Nt‐gh3</jats:italic> mRNA accumulates within a short time after auxin treatment, responds to very low concentrations of NAA (as little as 10<jats:sup>−9</jats:sup> M) and specifically to active auxins. <jats:italic>Nt‐gh3</jats:italic> mRNA is demonstrated to be one of the most relevant early molecular markers of primary auxin response.</jats:p>
  • Access State: Open Access