Description:
<jats:p>Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)<jats:italic>cis</jats:italic>Pt adduct binding of ER to estrogen response element (ERE)‐like sequences was examined. Three ERE‐like oligonucleotides with different affinities for ER and which include a GG in the linker sequence were designed in order to form a single central d(GpG)<jats:italic>cis</jats:italic>Pt adduct. Using electrophoretic mobility shift assay and Scatchard analysis, it was shown that the presence of a single d(GpG)<jats:italic>cis</jats:italic>Pt adduct in the linker sequence decreases the ER affinity for DNA. These results do not support a critical role of a DNA bend in the initial recognition of ERE by ER. Then, the platination of DNA outside of the ERE half‐sites decreases the interaction of ER with ERE.</jats:p>