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Media type:
E-Article
Title:
The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia
Contributor:
VICTOIR, K.;
BAÑULS, A. L.;
AREVALO, J.;
LLANOS-CUENTAS, A.;
HAMERS, R.;
NOËL, S.;
DE DONCKER, S.;
LE RAY, D.;
TIBAYRENC, M.;
DUJARDIN, J. C.
imprint:
Cambridge University Press (CUP), 1998
Published in:Parasitology
Language:
English
DOI:
10.1017/s0031182098002789
ISSN:
0031-1820;
1469-8161
Origination:
Footnote:
Description:
<jats:p>In the present study the gp63 gene locus was used as a target for genetic characterization of <jats:italic>Leishmania</jats:italic> parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus <jats:italic>Viannia</jats:italic>, namely <jats:italic>L</jats:italic>. (<jats:italic>V</jats:italic>.) <jats:italic>braziliensis</jats:italic>, <jats:italic>L</jats:italic>. (<jats:italic>V</jats:italic>.) <jats:italic>peruviana</jats:italic>, <jats:italic>L</jats:italic>. (<jats:italic>V</jats:italic>.) guyanensis and <jats:italic>L</jats:italic>. (<jats:italic>V</jats:italic>.) <jats:italic>lainsoni</jats:italic>: reference stocks of subgenus <jats:italic>Leishmania</jats:italic> were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a reference. On the one hand gp63–RFLP evidenced an extensive polymorphism and revealed specific markers for subgenus, species and geographical populations: congruence with MLEE was demonstrated statistically. The particular interest of gp63–RFLP was illustrated by infra-specific polymorphism, because of the possible relationship with phenotype diversity. On the other hand intra-genic amplification was less resolutive than gp63–RFLP, but also allowed discrimination of the 2 subgenera (PCR alone) and all the species tested in the subgenus <jats:italic>Viannia</jats:italic> (PCR–RFLP). PCR–RFLP presents an important operational advantage as it allows genetic characterization of minute amounts of parasites, using <jats:italic>Leishmania</jats:italic> specific primers. The polymorphism revealed by gp63–RFLP and PCR–RFLP illustrates the very high genomic and genetic plasticity of gp63 genes.</jats:p>