Published in:
Parasitology, 142 (2015) 6, Seite 783-790
Language:
English
DOI:
10.1017/s0031182014001851
ISSN:
0031-1820;
1469-8161
Origination:
Footnote:
Description:
SUMMARYTrichinella spiralisis an intracellular parasitic nematode of mammalian skeletal muscle, causing a serious zoonotic disease in humans and showing a high economic impact mainly in pig breeding. Serine proteinases ofT. spiralisplay important roles in the host–parasite interactions mediating host invasion. In this study, we have focused on newborn larvae (NBL-1), the first identified serine proteinase from the NBL stage ofT. spiralis.Five monoclonal antibodies (mAbs) directed against the C-terminal part of NBL1, were produced. These mAbs were IgG1κisotype and specifically recognized as a common motif of 10 amino acids (PSSGSRPTYP). Selected mAbs were further characterized using antigens from various developmental stages ofT. spiralis.Western blot revealed that selected mAbs reacted with the native NBL1 at Mr50 kDa in the adult and NBL mixed antigens and NBL stage alone. Indirect immunofluorescence analysis revealed that selected mAbs intensely stained only the embryos within the gravid females and the NBL. Thus, the produced mAbs are useful tools for the characterization of NBL1 as a major antigen ofTrichinellainvolved in the invasion of the host but also for the development of new serological tests with an early detection ofT. spiralisinfection.