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Rybar, Roman; Horakova, Jindra; Machatkova, Marie; Hanzalova, Katerina; Rubes, Jiri

Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robertsonian translocations: detection of translocations in embryos by fluorescence in situ hybridization

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  • Media type: E-Article
  • Title: Embryos produced in vitro from bulls carrying 16;20 and 1;29 Robertsonian translocations: detection of translocations in embryos by fluorescence in situ hybridization
  • Contributor: Rybar, Roman; Horakova, Jindra; Machatkova, Marie; Hanzalova, Katerina; Rubes, Jiri
  • imprint: Cambridge University Press (CUP), 2005
  • Published in: Zygote
  • Language: English
  • DOI: 10.1017/s0967199405003047
  • ISSN: 0967-1994; 1469-8730
  • Keywords: Cell Biology ; Developmental Biology
  • Origination:
  • Footnote:
  • Description: <jats:p>Robertsonian translocation rob(16;20) in the heterozygous state was discovered in a subfertile bull of the Czech Siemmental breed. A chromosomal analysis of its family has shown that this dicentric fusion is formed <jats:italic>de novo</jats:italic>. The present experiments were designed to detect rob(16;20) and determine its incidence for <jats:italic>in vitro</jats:italic> produced embryos, using fluorescence <jats:italic>in situ</jats:italic> hybridization (FISH) and rob(1;29) as a detection control. To characterize semen of both bulls with the rob translocations, their sperm was examined for DNA integrity by the sperm chromatin structure assay (SCSA). For <jats:italic>in vitro</jats:italic> fertilization of oocytes, spermatozoa from a rob(16;20) bull carrier (Czech Siemmental breed) and those from a rob(1;29) bull carrier (Charolais breed) were used. Embryos at the 6- to 8-cell stage were cultured in a vinblastine-supplemented medium for 17 h, and embryos at the blastocyst stage were cultured in a colcemide-supplemented medium for 4 h. The embryos were fixed in methanol and acetic acid with Tween-20. Painting probes for chromosomes 16 (Spectrum Green) and 20 (Spectrum Orange) and chromosomes 1 (Spectrum Orange) and 29 (Spectrum Green) were simultaneously hybridized. In the embryos derived from the rob(16;20) bull, the presence of this translocation was not detected. On the other hand, 52.5% of the embryos derived from the rob(1;29) bull were translocation carriers. There was no significant difference in the frequency of this translocation between early and advanced embryos.</jats:p>