Description:
AbstractN‐laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N‐laurylamine via the biotin ester of N‐hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse‐micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n‐octyl β‐D‐glucopyranoside.