You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni
Contributor:
Dugar, Gaurav;
Svensson, Sarah L.;
Bischler, Thorsten;
Wäldchen, Sina;
Reinhardt, Richard;
Sauer, Markus;
Sharma, Cynthia M.
Published:
Springer Science and Business Media LLC, 2016
Published in:
Nature Communications, 7 (2016) 1
Language:
English
DOI:
10.1038/ncomms11667
ISSN:
2041-1723
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>The widespread CsrA/RsmA protein regulators repress translation by binding GGA motifs in bacterial mRNAs. CsrA activity is primarily controlled through sequestration by multiple small regulatory RNAs. Here we investigate CsrA activity control in the absence of antagonizing small RNAs by examining the CsrA regulon in the human pathogen <jats:italic>Campylobacter jejuni.</jats:italic> We use genome-wide co-immunoprecipitation combined with RNA sequencing to show that CsrA primarily binds flagellar mRNAs and identify the major flagellin mRNA (<jats:italic>flaA</jats:italic>) as the main CsrA target. The <jats:italic>flaA</jats:italic> mRNA is translationally repressed by CsrA, but it can also titrate CsrA activity. Together with the main <jats:italic>C. jejuni</jats:italic> CsrA antagonist, the FliW protein, <jats:italic>flaA</jats:italic> mRNA controls CsrA-mediated post-transcriptional regulation of other flagellar genes. RNA-FISH reveals that <jats:italic>flaA</jats:italic> mRNA is expressed and localized at the poles of elongating cells. Polar <jats:italic>flaA</jats:italic> mRNA localization is translation dependent and is post-transcriptionally regulated by the CsrA-FliW network. Overall, our results suggest a role for CsrA-FliW in spatiotemporal control of flagella assembly and localization of a dual-function mRNA.</jats:p>