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Yelagandula, Ramesh; Bykov, Aleksandr; Vogt, Alexander; Heinen, Robert; Özkan, Ezgi; Strobl, Marcus Martin; Baar, Juliane Christina; Uzunova, Kristina; Hajdusits, Bence; Kordic, Darja; Suljic, Erna; Kurtovic-Kozaric, Amina; Izetbegovic, Sebija; Schaeffer, Justine; Hufnagl, Peter; Zoufaly, Alexander; Seitz, Tamara; Al-Rawi, Mariam; Ameres, Stefan; Baar, Juliane; Bauer, Benedikt; Beer, Nikolaus; Bergauer, Katharina; Binder, Wolfgang; [...]

Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq

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  • Media type: E-Article
  • Title: Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq
  • Contributor: Yelagandula, Ramesh; Bykov, Aleksandr; Vogt, Alexander; Heinen, Robert; Özkan, Ezgi; Strobl, Marcus Martin; Baar, Juliane Christina; Uzunova, Kristina; Hajdusits, Bence; Kordic, Darja; Suljic, Erna; Kurtovic-Kozaric, Amina; Izetbegovic, Sebija; Schaeffer, Justine; Hufnagl, Peter; Zoufaly, Alexander; Seitz, Tamara; Al-Rawi, Mariam; Ameres, Stefan; Baar, Juliane; Bauer, Benedikt; Beer, Nikolaus; Bergauer, Katharina; Binder, Wolfgang; [...]
  • imprint: Springer Science and Business Media LLC, 2021
  • Published in: Nature Communications
  • Language: English
  • DOI: 10.1038/s41467-021-22664-5
  • ISSN: 2041-1723
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, <jats:italic>saliva analysis by RNA sequencing</jats:italic>, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands of samples in parallel. SARSeq relies on next generation sequencing of multiple amplicons generated in a multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous and scalable assignment of reads to individual samples. We calibrate SARSeq on SARS-CoV-2 synthetic RNA, virions, and hundreds of human samples of various types. Robustness and sensitivity were virtually identical to quantitative RT-PCR. Double-blinded benchmarking to gold standard quantitative-RT-PCR performed by human diagnostics laboratories confirms this high sensitivity. SARSeq can be used to detect Influenza A and B viruses and human rhinovirus in parallel, and can be expanded for detection of other pathogens. Thus, SARSeq is ideally suited for differential diagnostic of infections during a pandemic.</jats:p>
  • Access State: Open Access