Published:
Springer Science and Business Media LLC, 2023
Published in:
Nature Communications, 14 (2023) 1
Language:
English
DOI:
10.1038/s41467-023-40065-8
ISSN:
2041-1723
Origination:
Footnote:
Description:
AbstractDNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT.