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Media type:
E-Article
Title:
Muscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
Contributor:
Dohmen, Richard G. J.;
Hubalek, Sophie;
Melke, Johanna;
Messmer, Tobias;
Cantoni, Federica;
Mei, Arianna;
Hueber, Rui;
Mitic, Rada;
Remmers, Dirk;
Moutsatsou, Panagiota;
Post, Mark J.;
Jackisch, Laura;
Flack, Joshua E.
Published:
Springer Science and Business Media LLC, 2022
Published in:
npj Science of Food, 6 (2022) 1
Language:
English
DOI:
10.1038/s41538-021-00122-2
ISSN:
2396-8370
Origination:
Footnote:
Description:
AbstractCultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-adipogenic progenitor cells (FAPs) are present in bovine muscle, and are transcriptionally and immunophenotypically distinct from satellite cells. These two cell types can be purified from a single muscle sample using a simple fluorescence-activated cell sorting (FACS) strategy. FAPs demonstrate high levels of adipogenic potential, as measured by gene expression changes and lipid accumulation, and can be proliferated for a large number of population doublings, demonstrating their suitability for a scalable cultured meat production process. Crucially, FAPs reach a mature level of adipogenic differentiation in three-dimensional, edible hydrogels. The resultant tissue accurately mimics traditional beef fat in terms of lipid profile and taste, and FAPs thus represent a promising candidate cell type for the production of cultured fat.