Khan, Nymul;
Maezato, Yukari;
McClure, Ryan S.;
Brislawn, Colin J.;
Mobberley, Jennifer M.;
Isern, Nancy;
Chrisler, William B.;
Markillie, Lye Meng;
Barney, Brett M.;
Song, Hyun-Seob;
Nelson, William C.;
Bernstein, Hans C.
Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
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Media type:
E-Article
Title:
Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
Contributor:
Khan, Nymul;
Maezato, Yukari;
McClure, Ryan S.;
Brislawn, Colin J.;
Mobberley, Jennifer M.;
Isern, Nancy;
Chrisler, William B.;
Markillie, Lye Meng;
Barney, Brett M.;
Song, Hyun-Seob;
Nelson, William C.;
Bernstein, Hans C.
Published:
Springer Science and Business Media LLC, 2018
Published in:
Scientific Reports, 8 (2018) 1
Language:
English
DOI:
10.1038/s41598-017-18630-1
ISSN:
2045-2322
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, <jats:italic>Halomonas</jats:italic> sp. HL-48 and <jats:italic>Marinobacter</jats:italic> sp. HL-58, to ask two fundamental scientific questions: 1) <jats:italic>how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth?</jats:italic> and 2) <jats:italic>how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions?</jats:italic> We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because <jats:italic>Marinobacter</jats:italic> HL-58 was supported by metabolites derived from <jats:italic>Halomonas</jats:italic> HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.</jats:p>