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Media type:
E-Article
Title:
Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
Contributor:
Paerl, Ryan W.;
Bertrand, Erin M.;
Rowland, Elden;
Schatt, Phillippe;
Mehiri, Mohamed;
Niehaus, Thomas D.;
Hanson, Andrew D.;
Riemann, Lasse;
Bouget, Francois-Yves
imprint:
Springer Science and Business Media LLC, 2018
Published in:Scientific Reports
Language:
English
DOI:
10.1038/s41598-018-24321-2
ISSN:
2045-2322
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>Almost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful <jats:italic>in vivo</jats:italic> B1 precursor for representatives of ubiquitous marine picoeukaryotic phytoplankton and <jats:italic>Escherichia coli</jats:italic> – drawing attention to cHET as a valuable exogenous micronutrient for microorganisms with ecological, industrial, and biomedical value. Comparative utilization experiments with the terrestrial plant <jats:italic>Arabidopsis thaliana</jats:italic> revealed that it can also use exogenous cHET, but notably, picoeukaryotic marine phytoplankton and <jats:italic>E. coli</jats:italic> were adapted to grow on low (picomolar) concentrations of exogenous cHET. Our results call for the modification of the conventional B1 biosynthesis model to incorporate cHET as a key precursor for B1 biosynthesis in two domains of life, and for consideration of cHET as a microbial micronutrient currency modulating marine primary productivity and community interactions in human gut-hosted microbiomes.</jats:p>