> Details

Picchi-Constante, Gisele Fernanda Assine; Guerra-Slompo, Eloise Pavão; Tahira, Ana Carolina; Alcantara, Monica Visnieski; Amaral, Murilo Sena; Ferreira, Arthur Schveitzer; Batista, Michel; Batista, Cassiano Martin; Goldenberg, Samuel; Verjovski-Almeida, Sergio; Zanchin, Nilson Ivo Tonin

Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells
  • Contributor: Picchi-Constante, Gisele Fernanda Assine; Guerra-Slompo, Eloise Pavão; Tahira, Ana Carolina; Alcantara, Monica Visnieski; Amaral, Murilo Sena; Ferreira, Arthur Schveitzer; Batista, Michel; Batista, Cassiano Martin; Goldenberg, Samuel; Verjovski-Almeida, Sergio; Zanchin, Nilson Ivo Tonin
  • Published: Springer Science and Business Media LLC, 2021
  • Published in: Scientific Reports, 11 (2021) 1
  • Language: English
  • DOI: 10.1038/s41598-021-01080-1
  • ISSN: 2045-2322
  • Keywords: Multidisciplinary
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p><jats:italic>Trypanosoma cruzi</jats:italic>—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architecture and chromatin structure along their complex life cycle which, combined with a remarkable set of reversible histone post-translational modifications, indicate that chromatin is also a target for control of gene expression and differentiation signals in these organisms. Chromatin-modifying enzymes have a direct impact on gene expression programs and DNA metabolism. In this work, we have investigated the function of <jats:italic>T. cruzi</jats:italic> histone deacetylase 4 (<jats:italic>Tc</jats:italic>HDAC4). We show that, although <jats:italic>Tc</jats:italic>HDAC4 is not essential for viability, metacyclic trypomastigote <jats:italic>Tc</jats:italic>HDAC4 null mutants show a thin cell body and a round and less condensed nucleus located very close to the kinetoplast. Sixty-four acetylation sites were quantitatively evaluated, which revealed H2AT85ac, H4K10ac and H4K78ac as potential target sites of <jats:italic>Tc</jats:italic>HDAC4. Gene expression analyses identified three chromosomes with overrepresented regions of differentially expressed genes in the <jats:italic>Tc</jats:italic>HDAC4 knockout mutant compared with the wild type, showing clusters of either up or downregulated genes. The adjacent chromosomal location of some of these genes indicates that <jats:italic>Tc</jats:italic>HDAC4 participates in gene expression regulation during <jats:italic>T. cruzi</jats:italic> differentiation.</jats:p>
  • Access State: Open Access