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Media type:
E-Article
Title:
Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles
Contributor:
Faddetta, Teresa;
Vassallo, Alberto;
Del Duca, Sara;
Gallo, Giuseppe;
Fani, Renato;
Puglia, Anna Maria
imprint:
Springer Science and Business Media LLC, 2022
Published in:
Scientific Reports, 12 (2022) 1
Language:
English
DOI:
10.1038/s41598-022-21002-z
ISSN:
2045-2322
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>Membrane vesicles (MVs) are spherical particles with nanoscale dimensions and characterized by the presence of diverse cargos, such as nucleic acids, proteins, lipids, and cellular metabolites. Many examples of (micro)organisms producing MVs are reported in literature. Among them, bacterial MVs are of particular interest because they are now considered as the fourth mechanism of horizontal gene transfer. <jats:italic>Streptomyces</jats:italic> bacteria are well-known for their ecological roles and ability to synthesize bioactive compounds, with <jats:italic>Streptomyces coelicolor</jats:italic> being the model organism. It was previously demonstrated that it can produce distinct populations of MVs characterized by different protein and metabolite cargos. In this work we demonstrated for the first time that MVs of <jats:italic>S. coelicolor</jats:italic> carry both DNA and RNA and that their DNA content represents the entire chromosome of the bacterium. These findings suggest that MV DNA could have a role in the evolution of <jats:italic>Streptomyces</jats:italic> genomes and that MVs could be exploited in new strain engineering strategies.</jats:p>