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Fraser, Alice J.; Greenland-Bews, Caitlin; Kelly, Daniel; Williams, Christopher T.; Bengey, Daisy; Buist, Kate; Clerkin, Karina; Finch, Lorna S; Gould, Susan; Kontogianni, Konstantina; Savage, Helen R; Thompson, Caitlin R; Wardale, Jahanara; Watkins, Rachel L; Wooding, Dominic; Allen, A. Joy; Body, Richard; Braybrook, Julian; Buckle, Peter; Clark, Eloïse; Dark, Paul; Davis, Kerrie; Gordon, Adam; Hayward, Gail; [...]

A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations

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  • Media type: E-Article
  • Title: A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations
  • Contributor: Fraser, Alice J.; Greenland-Bews, Caitlin; Kelly, Daniel; Williams, Christopher T.; Bengey, Daisy; Buist, Kate; Clerkin, Karina; Finch, Lorna S; Gould, Susan; Kontogianni, Konstantina; Savage, Helen R; Thompson, Caitlin R; Wardale, Jahanara; Watkins, Rachel L; Wooding, Dominic; Allen, A. Joy; Body, Richard; Braybrook, Julian; Buckle, Peter; Clark, Eloïse; Dark, Paul; Davis, Kerrie; Gordon, Adam; Hayward, Gail; [...]
  • Published: Springer Science and Business Media LLC, 2023
  • Published in: Scientific Reports, 13 (2023) 1
  • Language: English
  • DOI: 10.1038/s41598-023-30754-1
  • ISSN: 2045-2322
  • Origination:
  • Footnote:
  • Description: AbstractThe emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities.
  • Access State: Open Access