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Weber, Jan-Niklas; Minner-Meinen, Rieke; Behnecke, Maria; Biedendieck, Rebekka; Hänsch, Veit G.; Hercher, Thomas W.; Hertweck, Christian; van den Hout, Lena; Knüppel, Lars; Sivov, Simon; Schulze, Jutta; Mendel, Ralf-R.; Hänsch, Robert; Kaufholdt, David

Moonlighting Arabidopsis molybdate transporter 2 family and GSH-complex formation facilitate molybdenum homeostasis

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  • Media type: E-Article
  • Title: Moonlighting Arabidopsis molybdate transporter 2 family and GSH-complex formation facilitate molybdenum homeostasis
  • Contributor: Weber, Jan-Niklas; Minner-Meinen, Rieke; Behnecke, Maria; Biedendieck, Rebekka; Hänsch, Veit G.; Hercher, Thomas W.; Hertweck, Christian; van den Hout, Lena; Knüppel, Lars; Sivov, Simon; Schulze, Jutta; Mendel, Ralf-R.; Hänsch, Robert; Kaufholdt, David
  • Published: Springer Science and Business Media LLC, 2023
  • Published in: Communications Biology, 6 (2023) 1
  • Language: English
  • DOI: 10.1038/s42003-023-05161-x
  • ISSN: 2399-3642
  • Origination:
  • Footnote:
  • Description: AbstractMolybdenum (Mo) as essential micronutrient for plants, acts as active component of molybdenum cofactor (Moco). Core metabolic processes like nitrate assimilation or abscisic-acid biosynthesis rely on Moco-dependent enzymes. Although a family of molybdate transport proteins (MOT1) is known to date in Arabidopsis, molybdate homeostasis remained unclear. Here we report a second family of molybdate transporters (MOT2) playing key roles in molybdate distribution and usage. KO phenotype-analyses, cellular and organ-specific localization, and connection to Moco-biosynthesis enzymes via protein-protein interaction suggest involvement in cellular import of molybdate in leaves and reproductive organs. Furthermore, we detected a glutathione-molybdate complex, which reveals how vacuolar storage is maintained. A putative Golgi S-adenosyl-methionine transport function was reported recently for the MOT2-family. Here, we propose a moonlighting function, since clear evidence of molybdate transport was found in a yeast-system. Our characterization of the MOT2-family and the detection of a glutathione-molybdate complex unveil the plant-wide way of molybdate.
  • Access State: Open Access