> Details

Tang, Xinming; Yin, Guangwen; Qin, Mei; Tao, Geru; Suo, Jingxia; Liu, Xianyong; Suo, Xun

Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice
  • Contributor: Tang, Xinming; Yin, Guangwen; Qin, Mei; Tao, Geru; Suo, Jingxia; Liu, Xianyong; Suo, Xun
  • imprint: Springer Science and Business Media LLC, 2016
  • Published in: Scientific Reports
  • Language: English
  • DOI: 10.1038/srep29379
  • ISSN: 2045-2322
  • Keywords: Multidisciplinary
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>The surface antigen 1 of <jats:italic>Toxoplasma gondii</jats:italic> (TgSAG1) is a major immunodominant antigen and is widely considered an ideal candidate for the development of an effective recombinant vaccine against toxoplasmosis. <jats:italic>Eimeria tenella</jats:italic>, an affinis apicomplexan parasite with <jats:italic>T. gondii</jats:italic>, is a potential vaccine vector carrying exogenous antigens that stimulates specific immune responses. Here, we engineered TgSAG1 into <jats:italic>E. tenella</jats:italic> and obtained a stably transfected <jats:italic>E. tenella</jats:italic> line (Et-TgSAG1). We found TgSAG1 localized on the cell surface of Et-TgSAG1, which is similar to its native distribution in <jats:italic>T. gondii</jats:italic> tachyzoites. We immunized the chickens with Et-TgSAG1 orally and detected TgSAG1-specific immune responses, which partly reduced <jats:italic>T. gondii</jats:italic> infection. In the mouse model, we immunized the mice with Et-TgSAG1 sporozoites intraperitoneally and challenged them with <jats:italic>T. gondii</jats:italic> tachyzoites RH strain. We found that the mice immunized with Et-TgSAG1 showed a TgSAG1 specific Th 1-dominant immune response and a prolonged survival time compared with wild-type <jats:italic>E. tenella</jats:italic> and non-immunized mice. Collectively, our results demonstrated that Et-TgSAG1, utilized as a recombinant vaccine against toxoplasmosis, could be applied in both chickens and mice. Our findings also provide a promising persuasion for the development of transgenic <jats:italic>Eimeria</jats:italic> as vaccine vectors for use in birds and mammals.</jats:p>
  • Access State: Open Access