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Lenz, Florian; Zurek, Paul; Umlauf, Martina; Tozakidis, Iasson E. P.; Jose, Joachim

Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance

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  • Media type: E-Article
  • Title: Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance
  • Contributor: Lenz, Florian; Zurek, Paul; Umlauf, Martina; Tozakidis, Iasson E. P.; Jose, Joachim
  • imprint: Royal Society of Chemistry (RSC), 2020
  • Published in: Green Chemistry
  • Language: English
  • DOI: 10.1039/c9gc04166d
  • ISSN: 1463-9262; 1463-9270
  • Origination:
  • Footnote:
  • Description: <p>We evolved the thermophilic β-glucosidase <italic>Cs</italic>BglA to 150% improvement of <italic>k</italic><sub>cat</sub>/<italic>K</italic><sub>M</sub> at 55 °C, exerting a specific activity of 504 U mg<sup>−1</sup> and a <italic>K</italic><sub>M</sub>-value of 37.1 mM, making it the bacterial β-glucosidase with highest activity known so far.</p>