Contributor:
So, Peter T. C.;
Dong, Chen Y.;
Masters, Barry R.;
Berland, Keith M.
imprint:
Annual Reviews, 2000
Published in:
Annual Review of Biomedical Engineering, 2 (2000) 1, Seite 399-429
Language:
English
DOI:
10.1146/annurev.bioeng.2.1.399
ISSN:
1523-9829;
1545-4274
Origination:
Footnote:
Description:
<jats:p>▪ Abstract Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.</jats:p>