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Media type:
E-Article
Title:
Gas 6 promotes Axl-mediated survival in pulmonary endothelial cells
Contributor:
Healy, Aileen M.;
Schwartz, John J.;
Zhu, Xiahui;
Herrick, Brian E.;
Varnum, Brian;
Farber, Harrison W.
Published:
American Physiological Society, 2001
Published in:
American Journal of Physiology-Lung Cellular and Molecular Physiology, 280 (2001) 6, Seite L1273-L1281
Language:
English
DOI:
10.1152/ajplung.2001.280.6.l1273
ISSN:
1040-0605;
1522-1504
Origination:
Footnote:
Description:
We examined Gas 6-Axl interactions in human pulmonary artery endothelial cells (HPAEC) and in Axl-transduced HPAEC to test Gas 6 function during endothelial cell survival. We identified the 5.0-kb Axl, 4.2-kb Rse, and 2.6-kb Gas 6 mRNAs in HPAEC. Immunoprecipitation and Western blotting confirmed the presence of these proteins. Gas 6 is present in cell-associated and secreted fractions of growth-arrested HPAEC, independent of cell density. In addition, the Axl receptor is constitutively phosphorylated in growth-arrested cultures, and exogenous Gas 6 enhanced Axl phosphorylation threefold. Gas 6 added to growth-arrested HPAEC resulted in a significant increase in cell number (1.5 nM Gas 6 increased cell number 35%). Flow cytometry revealed that Gas 6 treatment resulted in 28% fewer apoptosing cells. Transduction of a full-length Axl cDNA into HPAEC resulted in 54% fewer apoptosing cells after Gas 6 treatment. Collectively, the data demonstrate antiapoptotic activities for Gas 6 in HPAEC and suggest that Gas 6 signaling may be relevant to endothelial cell survival in the quiescent environment of the vessel wall.