Saad, Normah;
Abdeshahian, Peyman;
Kalil, Mohd Sahaid;
Wan Yusoff, Wan Mohtar;
Abdul Hamid, Aidil
Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production byCunninghamella bainieri2A1 in Submerged Fermentation Using Response Surface Methodology
You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production byCunninghamella bainieri2A1 in Submerged Fermentation Using Response Surface Methodology
Contributor:
Saad, Normah;
Abdeshahian, Peyman;
Kalil, Mohd Sahaid;
Wan Yusoff, Wan Mohtar;
Abdul Hamid, Aidil
Published:
Hindawi Limited, 2014
Published in:
The Scientific World Journal, 2014 (2014), Seite 1-12
Language:
English
DOI:
10.1155/2014/280146
ISSN:
2356-6140;
1537-744X
Origination:
Footnote:
Description:
The locally isolated filamentous fungusCunninghamella bainieri2A1 was cultivated in a 5 L bioreactor to produce lipid and gamma-linolenic acid (GLA). The optimization was carried out using response surface methodology based on a central composite design. A statistical model, second-order polynomial model, was adjusted to the experimental data to evaluate the effect of key operating variables, including aeration rate and agitation speed on lipid production. Process analysis showed that linear and quadratic effect of agitation intensity significantly influenced lipid production process (P<0.01). The quadratic model also indicated that the interaction between aeration rate and agitation speed had a highly significant effect on lipid production (P<0.01). Experimental results showed that a lipid content of 38.71% was produced in optimum conditions using an airflow rate and agitation speed of 0.32 vvm and 599 rpm, respectively. Similar results revealed that 0.058 (g/g) gamma-linolenic acid was produced in optimum conditions where 1.0 vvm aeration rate and 441.45 rpm agitation rate were used. The regression model confirmed that aeration and agitation were of prime importance for optimum production of lipid in the bioreactor.