Description:
The glucan-Fe3O4magnetic nanoparticles were prepared by hydrothermal method. The mixture of FeCl2and glucan was stirred vigorously for half an hour under low temperature (15°C). KOH of 1 mol/L was dropwise added, slowly, into the solution until the pH to 12. Immediately, KNO3was added and the temperature was raised to 75°C for an hour. All the processes of Fe3O4crystal particles generation were under nitrogen. An atomic absorption spectrometry quantitative analysis method was built to determine the in vivo distribution of the glucan-Fe3O4magnetic nanoparticles in mice. The diameter of glucan-Fe3O4magnetic nanoparticles was about 25 nm and they were up taken by the liver primarily after intravenous administration via the tail.