Published in:
BioMed Research International, 2014 (2014), Seite 1-11
Language:
English
DOI:
10.1155/2014/914767
ISSN:
2314-6133;
2314-6141
Origination:
Footnote:
Description:
<jats:p>A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18<jats:sup>T</jats:sup>, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15–35°C, at pH 5.5–9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM<jats:italic>Streptomyces</jats:italic>agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus<jats:italic>Geodermatophilus</jats:italic>. The peptidoglycan contained<jats:italic>meso</jats:italic>-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H<jats:sub>4</jats:sub>) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C<jats:sub>16:0</jats:sub>and iso-C<jats:sub>15:0</jats:sub>and the unsaturated C<jats:sub>17:1</jats:sub><jats:italic>ω</jats:italic>8c and C<jats:sub>16:1</jats:sub><jats:italic>ω</jats:italic>7c. The 16S rRNA gene showed 97.4–99.1% sequence identity with the other representatives of genus<jats:italic>Geodermatophilus</jats:italic>. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18<jats:sup>T</jats:sup>is proposed to represent a novel species,<jats:italic>Geodermatophilus poikilotrophi</jats:italic>. Type strain is G18<jats:sup>T</jats:sup>(= DSM 44209<jats:sup>T</jats:sup>= CCUG 63018<jats:sup>T</jats:sup>). The INSDC accession number is HF970583. The novel R software package<jats:monospace>lethal</jats:monospace>was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.</jats:p>